筋電（EMG）と脳表EEGからマウスのREM睡眠期間を判断する方法の調査（３）
筋電（EMG）と脳表EEGからマウスのREM睡眠期間を判断する方法の調査（１） - 研究のヒホ
↑の続きです。
今回は測定したEMGと脳表EEG（大脳皮質EEG）からREM睡眠を判定する方法を調査してみました。
For both manual and automated staging, data were scored in 8-s epochs. In manual scoring, each epoch was staged by visual inspection as NREM, REM and wake by the following criteria: NREM sleep was characterized by high-amplitude EEG delta waves (0.5–4 Hz) with low EMG power, REM sleep was characterized by low-amplitude, high-frequency EEG theta waves (6–10 Hz) with very low EMG power, and wake was characterized by high and varying EMG power. Sunagawa, Genshiro A., et al.(2013)*1
理研の方の論文。日本人にもわかりやすい英語で書かれています。
REM : 小さいシータ帯のEEG＋とても小さいEMG
NonREM : 大きいデルタ帯のEEG＋小さいEMG
wake : 大きくて一様でないEMG
だそうです。なるほど。具体的にどのような波形のことを指すのかは、論文内のFigure 1. (A)を見るとわかりやすいかと思います。
WAKE, NREM, and REM sleep were manually scored using EEG/EMG in 10-second epochs during 24-hour baseline recordings. Sleep stages were determined as follows: epochs were scored as wake when the EMG amplitude ranged from activity slightly higher than baseline during quiet wakefulness to higher-amplitude activity during ambulation. EEG amplitude was low, with frequencies mostly above 10 Hz. NREM was characterized by high-amplitude delta (1-4 Hz). EMG was constant with low-amplitude activity. REM was scored when low-amplitude rhythmic theta waves (6-9 Hz) predominated, with the EMG remaining at baseline levels.
McShane, Blakeley B., et al.(2012)*2
前の論文とほとんど同じですね。wake時のEEGは10Hzが多いんですね。
24時間計測してベースラインを確保、それと比較しているようです。
Captured data were scored off-line manually to distinguish waking, REM sleep, and NREM sleep based on standard criteria including: (1) the amplitude and frequency of EEG; (2) the amplitude and frequency of EMG; and (3) the regularity of EEG/EMG.
Luo, Jie, et al.(2013)*3
JNの論文。具体的な方法が書いてなかった･･･
REM睡眠かどうか判定する方法がわかりました。もしかしたら、判定プログラムの作り方みたいなのを紹介します。
*1:Sunagawa, Genshiro A., et al. "FASTER: an unsupervised fully automated sleep staging method for mice." Genes to Cells 18.6 (2013): 502-518.
*2:McShane, Blakeley B., et al. "Assessing REM sleep in mice using video data." Sleep 35.3 (2012): 433.
*3:Luo, Jie, et al. "Increases in cAMP, MAPK activity, and CREB phosphorylation during REM sleep: implications for REM sleep and memory consolidation." The Journal of Neuroscience 33.15 (2013): 6460-6468.
